Highly efficient and non-toxic DNA isolation from very old bone marrow smears
نویسندگان
چکیده
منابع مشابه
I. Histochemical Staining of Bone Marrow Smears
Histochemical procedures for PMN granule enzymes were carried out on smears prepared from normal rabbit bone marrow, and the smears were examined by light microscopy. For each of the enzymes tested, azo dye and heavy metal techniques were utilized when possible. The distribution and intensity of each reaction were compared to the distribution of azurophil and specific granules in developing PMN...
متن کاملLymphoglandular bodies in bone marrow aspirate smears
which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. A 60-year-old man presented with fever for 2 weeks. Ultrasonography and contrast-enhanced abdominal computed tomography revealed retroperitoneal and mesenteric lymphadenopathy. Fine-needle aspiration cytology of a right inguinal lymph node showed a monotonou...
متن کاملPaediatric leukaemia DNA methylation profiling using MBD enrichment and SOLiD sequencing on archival bone marrow smears
BACKGROUND Acute Lymphoblastic Leukaemia (ALL) is the most common cancer in children. Over the past four decades, research has advanced the treatment of this cancer from a less than 60% chance of survival to over 85% today. The causal molecular mechanisms remain unclear. Here, we performed sequencing-based genomic DNA methylation profiling of eight paediatric ALL patients using archived bone ma...
متن کاملUnusual morphological cryoglobulin manifestations on blood and bone marrow smears.
Case report A 66-year-old female presented with a 3 years history of multiple ulcerations on the lower extremities of both legs. The diagnosis of necrotic angiodermitis had been previously proposed. The skin lesions were numerous (6 on right side and 7 on left side), purpuric, evolutive and painful, with width ranging from 0.5 to 4.5 centimeters. No adenopathy, neither liver or spleen enlargeme...
متن کاملThe Role of Bone Marrow Aspiration and Bone Marrow Biopsy in Diagnosis of Bone Marrow Metastases
Background: Bone marrow is the site of many malignant disorders and it is one of the common places for solid tumors to metastasize. Examination of the bone marrow aspirate and biopsy is a routine procedure performed for assessment of various conditions such as cytopenias, hematologic neoplasms, nonmalignant disorders and metastatic neoplasms. Methods: The patients were referred to the Hema...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Leukemia
سال: 1998
ISSN: 0887-6924,1476-5551
DOI: 10.1038/sj.leu.2401008